Results
| PMID | 11384649 |
| Gene Name | ESR1 |
| Condition | Endometriosis (Ovarian and Peritoneal) |
| Association |
Associated |
| Population size | 70 |
| Population details | 70 (33 peritoneal endometrial lesions, 37 ovarian endometriotic lesions) |
| Sex | Female |
| Associated genes | ER-alpha,ER-beta |
| Other associated phenotypes |
Ovarian endometriosis |
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Fertil Steril. 2001 Jun;75(6):1198-205. Matsuzaki, S| Murakami, T| Uehara, S| Canis, M| Sasano, H| Okamura, K Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan. matsuzaki@ob-gy.med.tohuko.ac.jp OBJECTIVE: To quantify and compare messenger RNA (mRNA) levels of ER-alpha and ER-beta among ovarian endometriotic cysts and red and black peritoneal endometriotic lesions. DESIGN: Prospective study. SETTING: University hospital. PATIENT(S): Patients with or without endometriosis. INTERVENTION(S): Samples of peritoneal (n = 33) and ovarian endometriotic lesions (n = 37) were obtained during laparoscopic surgery. Normal eutopic endometrial tissues and macroscopically normal peritoneal tissues were obtained as controls during or just after surgery. MAIN OUTCOME MEASURE(S): Expression of mRNA for ER-alpha and ER-beta, using a real-time reverse transcription (RT)-PCR assay, TaqMan RT-PCR, and nonradioactive in situ hybridization (ISH) techniques. RESULT(S): Both eutopic endometrium and endometriotic tissues showed predominantly higher levels of ER-alpha than ER-beta mRNA. Relative ratio of ER-alpha to ER-beta (ER-alpha/ER-beta) mRNA in red peritoneal lesions was significantly higher than in black lesions and ovarian endometriotic cysts. There was no significant difference in ER-alpha/ER-beta between proliferative eutopic endometrium and red peritoneal lesions. These results were confirmed by ISH analysis, which also revealed that the two estrogen receptors were localized in both epithelial and stromal cells of endometriotic tissues. CONCLUSION(S): The predominant expression of ER-alpha in both glandular epithelial and stromal cells may be essential for the development and growth of peritoneal and ovarian endometriosis. Mesh Terms: Adult| Endometriosis/*metabolism| Estrogen Receptor alpha| Estrogen Receptor beta| Female| Humans| In Situ Hybridization| Ovarian Diseases/*metabolism| Peritoneal Diseases/*metabolism| Prospective Studies| RNA, Messenger/metabolism| Receptors, |
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